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 Analysis of Photosynthetic Pigments from Blue-Green Algae

Analysis of Photosynthetic Pigments from Blue-Green Algae

Cyanobactera, also known as blue-green algae, obtain their energy by photosynthesis using sunlight as their energy source.  There are two classes of photosynthetic pigments in Cyanobactera.  The first class contains water-soluble proteins and the major protein is called Phycocyanin, which is blue.  The other classes of photosynthetic  pigments that include carotinoids and chlorophylls are small molecular weight molecules are are insoluble in water but soluble in organic solvents such as alcohol.  In this exercise, 16 students working pairs isolate and characterize both groups of pigments.  In part A of this exercise, students prepare a water-soluble extract from blue-green algae and show that it contains the single major protein Phycocyanin by electrophoresis.  They also determine the charge of this protein by comparing its electrophoretic mobility to the mobilities  of dyes with known charges.  In part B, they prepare an alcohol extract and analyze the smaller alcohol soluble pigments by thin layer chromatography in order to identify the chlorophylls and major carotenoid pigments.

Contents Included:
  • Complete instructions
  • Blue-green algae
  • Dye sample 1
  • Dye sample 2
  • Glycerol Solution
  • Thin-layer sheet
  • Chromatographic tube
  • 1.5mL tubes
  • Small transfer pipets
  • Small pipets
  • 5g agarose
  • TG buffer - contents make 3.5L of re-usable buffer
Accessories Needed:
  • Electrophoresis equipment
  • Methanol
  • Automatic pipetors (optional)
Product No. Description
UOM
Price
MBII29HS
Analysis of Photosynthetic Pigments from Blue-Green Algae
Each
$93.50

   
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Isaac Technologies Corp., 5420 Outer Drive, Windsor, ON N9A 6J3
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